背景：胶原蛋白已被长期用于改善皮肤的药物和营养产品中。 目的：我们评估高端胶原蛋白三肽对伤口愈合和皮肤恢复的有效性。 方法：通过体外测试模式，我们使用每毫升含1,10和100克的高端胶原蛋白三肽的浓缩物进行了HACAT（人永生化表皮细胞）细胞转移试验和胶原蛋白凝胶收缩试验。在此次实验性的研究中，八个健康志愿者被随意分为两组。受控组和实验组都接受点阵激光治疗。但实验组中四个主体接受四周，每天口服胶原蛋白肽3克的治疗。我们通过使用皮肤水分测试仪和皮肤弹性分析仪评估了每个病人在接受治疗前后表皮水分流失程度，并且我们也评估了病人的红斑指数。 结果：在不依赖剂量的情况下，细胞转移试验展示出高端胶原蛋白肽加快了伤口愈合。胶原蛋白凝胶收缩试验展示出当病人接受每升100毫克高端胶原蛋白肽治疗时，其伤口组织的伸缩性增强，但这结果跟受控组得到的结果区别不大。我们发现试验组中的个体在接受激光治疗后产生红斑的消退速度比受控组的快。此外，实验组中个体在激光治疗后皮肤补水恢复得比受控组好，并且试验组在治疗14天后，皮肤的弹性表现出显著地改善。 结论：胶原蛋白三肽治疗对于皮肤伤口愈合和点阵激光治疗后皮肤的恢复是一种有效和保守的治疗方法。
Effect of high advanced-collagen tripeptide on wound healing and skin recovery after fractional photothermolysis treatment. BACKGROUND: Collagens have long been used in pharmaceuticals and food supplements for the improvement of skin. AIM:We evaluated the efficacy of high advanced-collagen tripeptide (HACP) on wound healing and skin recovery. METHODS:Using an in vitro model, we performed HaCaT cell migration assays and collagen gel contraction assays using HACP concentrations of 1, 10 and 100 μg/mL. In this pilot study, eight healthy volunteers were randomly divided into two groups. Both the control and experimental groups received fractional photothermolysis treatment, but in the experimental group, four subjects received 3 g/day of oral collagen peptide (CP) for 4 weeks. To assess transepidermal water loss in each patient before and after the treatment, we used a Corneometer and a Cutometer, and we also assessed the patient's Erythema Index. RESULTS:The cell migration assay showed that HACP enhanced wound closure, but not in a dose-dependent manner. The collagen gel contraction assay showed increased contractility when patients were treated with 100 μg/mL HACP, but the results were not significantly different from those of controls. We found that post-laser erythema resolved faster in the experimental group than in the control group (P < 0.05). In addition, the recovery of skin hydration after fractional laser treatment was greater in the experimental group than in the control group by day 3 (P < 0.05), and the experimental group showed significantly improved post-treatment skin elasticity compared with the controls by day 14 (P < 0.05). CONCLUSIONS:Collagen tripeptide treatment appears to be an effective and conservative therapy for cutaneous wound healing and skin recovery after fractional photothermolysis treatment.